Targeted genomic rearrangements using CRISPR/Cas technology

Genomic rearrangements are frequently observed in cancer cells but have been difficult to generate in a highly specific manner for functional analysis. Here we report the application of CRISPR/Cas technology to successfully generate several types of chromosomal rearrangements implicated as driver events in lung cancer, including the CD74-ROS1 translocation event and the EML4-ALK and KIF5B-RET inversion events. Our results demonstrate that Cas9-induced DNA breaks promote efficient rearrangement between pairs of targeted loci, providing a highly tractable approach for the study of genomic rearrangements

Reproducibility of a Titanium Plasma Vacuum Spark Discharge

The results of an extensive operation of a Vacuum Spark plasma using Titanium electrodes in a 120 ns 150 kA discharge are presented. The hot spots are found to form with a regular spacing in a zippering Z-pinch plasma, which forms close to the cathode and extends to approximately two thirds of the anode separation over a period of a few ns. The axis of the discharge is well defined by an initial plasma from a Nd:YAG laser focussed onto the cathode electrode surface. The statistics of the formation of the hot spots are given for the life of one anode electrode. Between one and three hotspots form and the favored positions are at 1.5 and 3.0 mm from the cathode and the strongest emission, as observed in a filtered X-ray pinhole camera, comes from the hot spot closest to the cathode. The emission spectra resolved between 50 and 350 \AA shows a wide range of Ti ionization which allows the temperatures of the anode blow off plasma, the Z-pinch and the hot spot plasma to be distinguished. These results are compared with filtered PIN diode signals and filtered pinhole images.Comment: 5 pages, 4 figure

Unified radio and network control across heterogeneous hardware platforms

Experimentation is an important step in the investigation of techniques for handling spectrum scarcity or the development of new waveforms in future wireless networks. However, it is impractical and not cost effective to construct custom platforms for each future network scenario to be investigated. This problem is addressed by defining Unified Programming Interfaces that allow common access to several platforms for experimentation-based prototyping, research, and development purposes. The design of these interfaces is driven by a diverse set of scenarios that capture the functionality relevant to future network implementations while trying to keep them as generic as possible. Herein, the definition of this set of scenarios is presented as well as the architecture for supporting experimentation-based wireless research over multiple hardware platforms. The proposed architecture for experimentation incorporates both local and global unified interfaces to control any aspect of a wireless system while being completely agnostic to the actual technology incorporated. Control is feasible from the low-level features of individual radios to the entire network stack, including hierarchical control combinations. A testbed to enable the use of the above architecture is utilized that uses a backbone network in order to be able to extract measurements and observe the overall behaviour of the system under test without imposing further communication overhead to the actual experiment. Based on the aforementioned architecture, a system is proposed that is able to support the advancement of intelligent techniques for future networks through experimentation while decoupling promising algorithms and techniques from the capabilities of a specific hardware platform

Anomalous frequency and intensity scaling of collective and local modes in a coupled spin tetrahedron system

We report on the magnetic excitation spectrum of the coupled spin tetrahedral system Cu$_{2}$Te$_{2}$O$_{5}$Cl$_{2}$ using Raman scattering on single crystals. The transition to an ordered state at T$_{N}^{Cl}$=18.2 K evidenced from thermodynamic data leads to the evolution of distinct low-energy magnetic excitations superimposed by a broad maximum. These modes are ascribed to magnons with different degree of localization and a two-magnon continuum. Two of the modes develop a substantial energy shift with decreasing temperature similar to the order parameter of other Neel ordered systems. The other two modes show only a negligible temperature dependence and dissolve above the ordering temperature in a continuum of excitations at finite energies. These observations point to a delicate interplay of magnetic inter- and intra-tetrahedra degrees of freedom and an importance of singlet fluctuations in describing a spin dynamics.Comment: 7pages, 6figures, 1tabl

Singlet superfield extension of the minimal supersymmetric standard model with Peccei-Quinn symmetry and a light pseudoscalar Higgs boson at the LHC

Motivated by the mu-problem and the axion solution to the strong CP-problem, we extend the MSSM with one more chiral singlet field $X_e$. The underlying PQ-symmetry allows only one more term $X_e H_u H_d$ in the superpotential. The spectrum of the Higgs system includes a light pseudoscalar $a_X$ (in addition to the standard CP-even Higgs boson), predominantly decaying to two photons: $a_X \to \gamma \gamma$. Both Higgs bosons might be in the range accessible to current LHC experiments.Comment: 5 pages with 3 figure

The Gaugino Code

Gauginos might play a crucial role in the search for supersymmetry at the Large Hadron Collider (LHC). Mass predictions for gauginos are rather robust and often related to the values of the gauge couplings. We analyse the ratios of gaugino masses in the LHC energy range for various schemes of supersymmetry breakdown and mediation. Three distinct mass patterns emerge.Comment: 42 pages, Latex; a discussion of deflected anomaly mediation added, references adde

Axionic dark energy and a composite QCD axion

We discuss the idea that the model-independent (MI) axion of string theory is the source of quintessential dark energy. The scenario is completed with a composite QCD axion from hidden sector squark condensation that could serve as dark matter candidate. The mechanism relies on the fact that the hidden sector anomaly contribution to the composite axion is much smaller than the QCD anomaly term. This intuitively surprising scenario is based on the fact that below the hidden sector scale $\Lambda_h$ there are many light hidden sector quarks. Simply, by counting engineering dimensions the hidden sector instanton potential can be made negligible compared to the QCD anomaly term.Comment: 9 pages, 7 figure